NIH WORKSHOP

The Conference Organizing Committee is pleased to announce a partnership with our colleagues at the National Institutes of Health (NIH) to host a multi-day workshop in conjunction with CALCON 2011. The jointly organized workshop on “Global and Multi-Method Analysis of Macromolecular Interactions with SEDPHAT” is scheduled to convene over a three-day period immediately preceding the conference (i.e., Friday thru Sunday, June 10–12, 2011).

The instructional staff is comprised of renowned experts who will participate in both the Workshop and the CALCON 2011 Scientific Program. Hosting both events at a single location allows us to combine resources and offer an all-inclusive Conference/Workshop Registration Fee of $ 995 and $ 500 for participants and students, respectively.

We encourage conferees to avail themselves of this unique opportunity to participate in the first jointly organized workshop on Global Analysis of Macromolecular Interactions amidst the scenic splendor of Turtle Bay Resort on the North Shore of Oahu.

Agenda

The workshop is aimed at researchers with prior experience in experimental biophysical methods and will review strategies for:

Isothermal titration calorimetry (ITC)
Hydrodynamic and thermodynamic analysis of sedimentation equilibrium and sedimentation velocity analytical ultracentrifugation (AUC)
Dynamic light scattering (DLS)
Fluorescence spectroscopy (anisotropy and quenching isotherms),
Optical biosensing (SPR) for the characterization of biological macromolecules in solution and their interactions.

The workshop will introduce experimental aspects of ITC, AUC, and DLS while reviewing basic operation of the instruments, selection of buffers and detection systems, and experimental design considerations dependent on the system under study.

The workshop lectures will cover advanced topics of theory, data analysis, and experimental setup accompanied by practical computer exercises using the SEDPHAT software platform.

A unique feature of the workshop is the dedicated one-on-one sessions in which each participant's data is analyzed and discussed. Additional information regarding specific details of the workshop and SEDPHAT are available by visiting the following link:

Topics

Overview of ITC/AUC/DLS techniques and experimental design considerations
Global analysis concepts with SEDPHAT
General aspects of non-linear regression and statistical error analysis
Overview of association models and fitting options
ITC analysis for single and multiple titrations
ITC analysis of ternary interactions
SPR analysis in EVILFIT: continuous distributions of rate and affinity constants
Dynamic light scattering analysis
General isotherm analysis with SEDPHAT (AUC, spectroscopic methods, SPR)
Overview of sedimentation velocity analysis with SEDFIT
Sedimentation coefficient and molar mass distributions
Analysis of self-associating systems by c(s), isotherms, and direct boundary modeling
Analysis of hetero-associating systems by sedimentation velocity
Multi-signal sedimentation velocity analysis for heterogeneous interactions
Sedimentation equilibrium analysis with multi-wavelength data and soft mass conservation models

Instructors

Prof. Chad Brautigam
Biochemistry Department, UT Southwestern Medical School, Dallas, TX

Dr. Rodolfo Ghirlando
NIDDK, National Institutes of Health, Bethesda, MD

Dr. Peter Schuck
NIBIB, National Institutes of Health, Bethesda, MD

Dr. Huaying Zhao
NIBIB, National Institutes of Health, Bethesda, MD

Workshop Registration

The Conference Organizing Committee has established a time-dependent fee schedule to reward Conferees who register for the NIH Workshop early. The Registration Fee and Deadline Schedule for Workshop Participants and Students is as follows:

Registration	Participant	Student	Deadline
EARLY	$ 400	$ 250	April 15, 2011
REGULAR	$ 500	$ 300	May 1 , 2011
LATE	$ 600	$ 350	June 1 , 2011

Conferees are encouraged to complete their Workshop Registration online via direct debiting of Mastercard, Visa, or American Express at the following link:

On-line Registration Form

An alternative option is to download and submit the manual registration form to the Secretary-Treasurer accompanied by credit card payment (Mastercard, Visa, American Express) or an official bank check payable to The Calorimetry Conference [CALCON 2011 REGISTRATION FORM].

Please contact calcon@rci.rutgers.edu in the event you have questions

regarding Conference or Workshop Registration.